Wnt Signaling Research

Wnt signaling is essential for axis formation. By overexpression of wnt8 or b-catenin, we can induce secondary axes in frogs and create tadpoles with two body axes.

Wnt Signaling

Canonical Wnt signaling requires that b-catenin enter the nucleus in order to activate wnt target genes. By tagging b-catenin with GFP, we can monitor the localization of b-catenin at the cell cortex or in the nucleus.
The image at left depicts nuclear localization of beta-catenin in stage 10 xenopus embryo. J. Griffin.