For New Lab Members
Required Online Training and Health Screening
The following steps need to be taken to be added to our Lab Animal Protocol. Download a copy of these instructions here.
Online Forms and Training:
After logging in, click on Browse Couses & Forms in the Left Hand navigation bar.
Select “Animal Care and Use.” Click the “Courses” box, under “ All Animal Handlers: Mandatory Training.”
The first form, “IACUC Personnel Qualifications”, will be submitted by the Khokha lab. Please email Maura with your Net ID for the form (firstname.lastname@example.org).“Medical Surveillance Program for Animal Handlers.” Click the link to download the Medical Surveillance Form. Print and fill in this form.
Important: Make a note on the form: “Clearance requested to work with Aquatic Species only”.
Fax this form to the number on the form (Employee Health).
Complete the listed courses:
Regulatory Training for Animal Care & Use
YARC facility Training Part I.
Please let Maura know after you have completed these courses and received notification that your Medical Surveillance has been cleared. I will submit your application to be added to our Animal Protocol at this time.
Please also complete Laboratory Chemical Safely and Biosafety Part I courses, found at:
- Browse Couses & Forms >Environmental Health and Safety > Chemical Safety > Laboratory Chemical Safety Web Based
- Browse Couses & Forms >Environmental Health and Safety > Biological Safety >Biosafety - Part 1 web-based
If you require access to the animal housing areas (tropicalis and laevis rooms), please complete the following you after you have been added to our Animal Protocol(Maura will let you know when that happens). :
Sign up for the YARC Facility Training Part II on the Yale Training website. This is a 15 - 20 minute tour of our X. laevis colony (Room WWW B27) room offered every other Thursday.
You will need to have a brief tour of our X. tropicalis facility also, which can be arranged with Maura.
IACUC Policies and Procedures to Review
The Yale Animal Care and Use Committee (IACUC) oversees all animal related research. They have published Policies, Procedures and Guidelines which need to be followed when working with animals. Failure to comply can result in citations and even closure of the lab in extreme circumstances.
To review the sections of these policies pertinent to our research, please go to this link:
Read the following sections:
Guidelines: General Health - All species
Policies: Analgesia- Amphibians and Fish table
Policies: Euthanasia - Guidelines and Procedures: sec 4414.10
Policies: Expired Materials
Khokha Lab Protocol
Our Animal Use Protocol (AUP) covers all aspects of animal use in our lab, such as who is allowed to work with the animals, what experiments we can do, how many animals we can keep, husbandry policies, and a host of other things. In order to gain some familiarity with the AUP, please read over the blue highlighted sections of the 2015 protocol, located in a binder named "AUP 2012-2015", on the top shelf over Maura desk.
Lab Rules and Safety
The following General Lab Safety and Etiquette guidelines are important to know. You will receive an in-person review of these rules, but please read them over here:
EH+S requires gloves, labcoat, close toed shoes and safety glasseswhen working at the bench.
More in depth information on Yale Policy on Personal Protective Equipment can be found here:
It is important to know where to dispose of certain types of waste in the lab. Biohazard bins vs sharps boxes vs trash all take different kind of waste:
Glass pipettes: in plastic sharps box, NOT trash or biohazard bin
Pipette tips: in biohazard bin or plastic sharps box, NOT trash
Serological pipettes in plastic tray on counter near sink
broken, non-hazardous glass in "Broken Glass" box by 425 lab door
Volatile or hazardous chemicals in lab are generally separated into secondary containers by type (acids, bases etc.). Hazardous chemicals are kept in storage cabinets and should be used in the fume hood with gloves, lab coat and safety goggles. You will receive further training on this topic when you complete the online Chemical Safety course (link given next section), but be aware it is important to work with these chemicals according to guidelines. Don't hesitate to ask if you are unsure about use of any chemical.
Agar waste bucket
Please crush or nest agar cups in the agar waste bucket to save space. Also, always wear gloves when handling Ethidium bromide and avoid inhaling fumes.
Please make a note on whiteboard near door of room 425 when running low on something.
Hands-on training in Lab
You will be trained hands on in lab to work with the species. This training will include:
Lab Housing rules for the species
General health, use and care
Other procedures relevant to your project