Request Frogs

Inbred Strains

We have a number of frogs availble for distribution. To obtain more information, pictures of embryos, or frogs, please send your requests to Mustafa Khokha (mustafa.khokha@yale.edu).

Inbred lines
NameAvailable AsDescriptionGeneration
NigerianMating PairThese are inbred Nigerian X. tropicalis kindly provided to the Harland lab by Rob Grainger, then bred in the lab. We have identified background mutations in these animals - grinch and curly. However, we have identified a set of animals without these background mutations which are available for distribution
Nigerian "Superman"MalesThese are inbred animals derived from a Nigerian male who showed very early nuptial pads and had very good fertilization rates. We have inbred a line arising from "Superman", and selected for early nuptial pads/maturation/good fert rates.
Nigerian "Lois"FemalesThese females are also derived from the Superman Nigerian male. They are very large robust females who give large numbers of high quality eggs.
Ivory Coast B (ICB) "blue" or "Axanthic"FrogletsICB strain frogs appear to be richly polymorphic compared to Nigerian. In addition, they have a pigmentation defect, making them easy to distinguish.F8
Population AFrogletsThese animals were originally obtained from Rob Grainger and then brought to the Harland lab via David Wright.
Natural Mutants
Beaverfroglets
Curlyfroglets
Grinchfroglets
Bubbletroublefroglets
Transgenic Pigment Mutants
No Privacy (NOP)froglets
Axanthic/No Privacy (Axa/NOP)froglets
NOBfroglets
TyrosinasefrogletsThese frogs derive from tryosinase KO embryos created using CRISPR.
Tyrosinase/AxanthicfrogletsThese frogs came from tryosinase KO embryos created using CRISPR. Subsequent generations were crossed with the Ivory coast "B" Axanthic line (see above) which have a severe reduction in xanthophores. Also have some which were Tyr inn into Axa?
Other Transgenics
Triplefroglets
Flk-T2DfrogletsThis line has tissue specific Flk expression(GFP in heart and vasculature). We have lines created using both Tol1 and Tol2 technology.
n-Tub-T2DfrogletsThis line has tissue specific n-tubulin expression(GFP in neural tissue), created using Tol2 technology.
Brainbow1.0H-RFPfrogletsThese animals express ubiquitous RFP initially, but can be manipulated using Flp recombinase to allow expression of other fluorophores in subsets of tissues. Please see "put link to Brainbow information here" for more information on this technology. also put link to Jon for more info? These animals were created using Tol 1 technology.
pMLC1V-RFPfrogletsThese animals express tissue specific myosin light chain RFP(heart, facial muscle). They were created using Tol 1 technology.
Piggy-BacfrogletsThese animals express very intense ubiquitous RFP. They arise from embryos injected using Piggy-Bac technology, and likely contain a number of transposon concatemers.

-Tim Grammer, Mustafa Khokha, Maura Lane